![]() Transcriptional profiling analyses revealed that cellulase gene expression levels were maintained at high levels for 56 h in the Δ stk-12 mutant, compared to only 8 h in the wild-type (WT) strain. These results suggested that stk-12 plays a critical role as a brake to turn down the transcription of cellulase genes to repress the overexpression of hydrolytic enzymes and prevent energy wastage. We observed that the loss of stk-12 (NCU07378) caused a dramatic increase in cellulase production and an extended period of high transcript abundance of major cellulase genes. We systematically screened a protein kinase deletion set in the filamentous fungus Neurospora crassa to search for mutants exhibiting aberrant expression patterns of cellulase genes. However, the mechanisms underlying this bell-shaped expression pattern are unclear. When fungi are exposed to cellulose, the transcript levels of cellulase genes rapidly increase and then decline. Cellulolytic fungi have evolved a complex regulatory network to maintain the precise balance of nutrients required for growth and hydrolytic enzyme production.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |